產(chǎn)品屬性

• Guinea pig-anti-Sox10 polyclonal antibody

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  Catalog  No: OB-PGP113

  RRID: AB_2934230

  Host Species: Guinea pig polyclonal antibodies

  Important Notice: Antigen retrieval is recommended before IHC/IF staining.

  Gene Synonyms: Sox10, SRY-box transcription factor 10

  Species Reactivity: Mouse, rat, human, chicken, dog; Others have not been tested

  Applications: IF/IHC-Fr: 1:500~2000     ICC: 1:500     WB: 1:2000~5000     IHC-P: 1:200~1000

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  Antigen Retrieval: For an alternative approach, tissue sections could be incubated in 1x Citrate antigen retrieval solution for 30 min at 85~95°C and cool down to room temperature before next procedure.

  Important Notice: Usually, it is easier to get a positive IF/IHC signal in sections of juvenile animal tissues than that of adults. This antibody  does not recognize other SoxE proteins (Sox8 and Sox9).

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  Immunogen: Mouse Sox10 peptide fused to GST.      

  Purification: Affinity purification

  General Notes: FOR RESEARCH USE ONLY

  Storage Buffer: pH7.4, PBS, 55mM Tris, 50mM Glycine, ~40% Glycerol, 0.1% potassium sorbate

  Storage: Shipped at 4°C. Store at -20/4°C for months and -80°C for years. Avoid freeze/thaw cycle.

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  Background: SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors. SOX10 is a specific pan-oligodendrocyte marker in CNS, and also highly expressed in skin melanocytes, neural crest cells and Schwann cells. Conditional ablation of Sox10 gene in mice leads to myelin defects in both the CNS and PNS [3, 4]. Conventional null mutants lack peripheral glial cells, melanocytes, and autonomic and enteric neurons, adrenal medulla, and die neonatally or sooner [5-8].

  [1] Stolt CC, et al. Genes Dev. 2002; DIO: 10.1101/gad.215802

  [2] Schreiner S, et al. Development. 2007; DIO: 10.1242/dev.003350

  [3] Finzsch M, et al. J Cell Biol. 2010; DIO: 10.1083/jcb.200912142

  [4] Hornig J, et al. PLoS Genet. 2013; DIO: 10.1371/journal.pgen.1003907

  [5] Reiprich S, et al. Mol Biol Cell. 2008; DIO: 10.1091/mbc.E07-08-0782

  [6] Wegner M, et al. Trends Neurosci. 2005; DIO: 10.1016/j.tins.2005.08.008

  [7] Britsch S, et al. Genes Dev. 2001; DIO: 10.1101/gad.186601

  [8] Harris ML, et al. PLoS Genet. 2013; DIO: 10.1371/journal.pgen.1003644

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  Key Words: oligodendrocyte, myelin, oligodendrocyte progenitor cell, melanocyte, Schwann cell, adrenal medulla, peripheral nervous system, central nervous system, transcriptional factor

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