產(chǎn)品屬性

Guinea pig-anti-Parvalbumin antibody 

Catalog No: OB-PGP005

RRID: AB_2938894

Host Species: Guinea pig polyclonal antibodies

Important Notice: Antigen retrieval is recommended.

Gene Synonyms: Pvalb, parvalbumin, Parv, PV, Pva

Species Reactivity: Mouse, rat, human, dog; Predict to work in primates

Applications: IF/IHC-Fr: 1:500~2000   IHC-P: 1:500~2000   WB: 1:2000~5000

Antigen Retrieval: For an alternative approach, tissue sections could be incubated in 1x Citrate antigen retrieval solution for 30 min at 85~95°C and cool down to room temperature before next procedure.

Immunogen: Mouse Pavlb peptide fused to GST.

Purification: Affinity purification

General Notes: FOR RESEARCH USE ONLY

Storage Buffer: pH7.4, PBS, 55mM Tris, 50mM Glycine, ~40% Glycerol, 0.1% potassium sorbate

Storage: Shipped at 4°C. Store at -20/4°C for months and -80°C for years. Avoid freeze/thaw cycle.

Background: Parvalbumins are small calcium-binding proteins, members of the EF-hand protein family, that are expressed in vertebrates. Particularly abundant in certain skeletal muscle fibers and neurons, parvalbumins function primarily as mobile cytosolic Ca2+ buffers. They influence the duration of intracellular Ca2+ signals and play key roles in muscle relaxation following contraction and neuronal recovery following excitation [1]. Mice homozygous for deletion of this marker show slower cotraction-relaxation of fast twitch muscle and increased force generation. Abnormalities are also reported in Purkinje cell morphology [2, 3]. In the neocortex region, expressing cells represent the largest category of inhibitory interneurons (~30%–50%) [4, 5].

[1] Pauls TL, et al. Biochim Biophys Acta. 1996; DIO: 10.1016/0167-4781(95)00221-9

[2] Chen G, et al. Neuroscience. 2006; DIO: 10.1016/j.neuroscience.2006.06.008

[3] Schwaller B, et al. Am J Physiol. 1999; DIO: 10.1152/ajpcell.1999.276.2.C395

[4] Rudy B, et al. Dev Neurobiol. 2011; DIO: 10.1002/dneu.20853

[5] Nichols J, et al. J Physiol. 2018; DIO: 10.1113/jp275393

Key Words: Cortical interneurons, basket cells, inhibitory interneurons, GABAergic interneurons